Cell Imaging and Analysis (CIA) Facilities and Services Core

Core members left to right: Tamara Fraley, Danielle King, Jeff Greenwood (Core Director), Christiane Löhr, and Nancy Kerkvliet

The mission of the Cell Imaging and Analysis Facility Core is to provide access to state-of-the-art instrumentation and expertise in confocal microscopy, flow cytometry, cell sorting, and histopathology.

Imaging is an indispensable mode of inquiry for researchers studying the mechanisms by which environmental toxins affect tissues, cells, and subcellular organelles. Recent advances such as fluorescent probes, green fluorescent protein (GFP), confocal optics, two-photon optics, and photoelectronic detectors have improved our ability to image complex tissues. They have enabled us to visualize single molecules and to monitor molecular interactions in live cells. Cost and complexity issues make it impractical for individual investigators to obtain state-of-the-art imaging technologies. Therefore, the need exists for an organized group of specialists with expertise in imaging technologies to acquire the instrumentation, master the applications, and help individual investigators apply the technology to answer specific research questions.

NEW: The laser capture microdissection system is open for business. 

Services

We support EHSC Investigators to accomplish these tasks:

• Determine the damage resulting from exposure to environmental toxins and other treatments by providing expert pathological analysis from an experienced Veterinary Pathologist.
• Acquire high-resolution images of zebrafish and other models by providing instrumentation, analysis software, infrastructure, and technical assistance.
• Quantify the activation state of signal transduction pathways by developing advanced methodology using flow cytometry, microscopy, and mass spectrometry.
• Isolate specific cell populations from cell culture, blood, and tissue by providing instrumentation and technical assistance.

Click the links below for more information on the technologies for cell imaging, flow cytometry and cell sorting and the pathological analysis of carcinogenetic studies.

• Cell Imaging and Culture
  
• Flow Cytometry and Cell Sorting
• Tissue Analysis and Pathology

Note: Any manuscript submitted for publication that contains data generated in these facilities should contain an acknowledgement of the services provided by the "Cell Imaging and Analysis Facilities and Services Core of the Environmental Health Sciences Center, Oregon State University, grant number P30 ES00210, National Institute of Environmental Health Sciences, National Institutes of Health."

Publications

2009

Bajaj G, Zhang Y, Schimerlik MI, Hau AM, Yang J, Filtz TM, Kioussi C, Ishmael JE (2009) N-Methyl-D-aspartate Receptor Subunits Are Non-myosin Targets of Myosin Regulatory Light Chain. J. Biol. Chem. 284:1252-66.

Castro DJ, Löhr CV, Fischer KA, Waters KM, Webb-Robertson BJ, Dashwood RH, Bailey GS, Williams DE (2009) Identifying Efficacious Approaches to Chemoprevention with Chlorophyllin, Purified Chlorophylls and Freeze-dried Spinach in a Mouse Model of Transplacental Carcinogenesis. Carcinogenesis 30:315-20.

Jang HS and Greenwood JA (2009) Gylcine-rich region regulates cysteine-rich protein 1 binding to actin cytoskeleton. Biochem. Biophys. Res. Commun., 380:484-88.

Kerkvliet NI (2009) AHR-mediated immunomodulation: The role of altered gene transcription. Biochem. Pharmacol. 77:746-60.

2008

Cassina P, Cassina A, Pehar M, Castellanos R, Gandelman M, de Leon A, Robinson KM, Mason RP, Beckman JS, Barbeito L, and Radi R (2008) Mitochondrial dysfunction in SOD1G93A-bearing astrocytes promotes motor neuron degeneration: prevention by mitochondrial-targeted antioxidants. J Neurosci. 28:4115-22.

Castro DJ, Yu Z, Lohr CV, Pereira CB, Giovanini JN, Fischer KA, Orner GA, Dashwood RH, and Williams DE (2008) Chemoprevention of dibenzo[a,l]pyrene transplacental carcinogenesis in mice born to mothers administered green tea: primary role of caffeine. Carcinogenesis. 29:1581-6.

Castro DJ, Baird WM, Pereira CB, Giovanini J, Löhr CV, Fischer KA, Yu Z, Gonzalez FJ, Krueger SK, Williams DE (2008) Fetal mouse Cyp1b1 and transplacental carcinogenesis from maternal exposure to dibenzo(a,l)pyrene. Cancer Prev. Res. (Phila Pa) 1:128-34.

Castro DJ, Löhr CV, Fischer KA, Pereira CB, Williams DE (2008) Lymphoma and lung cancer in offspring born to pregnant mice dosed with dibenzo[a,l]pyrene: the importance of in utero vs. lactational exposure. Toxicol. Appl. Pharmacol. 233:454-8.

Funatake CJ, Marshall NB, Kerkvliet NI (2008) 2,3,7,8-Tetrachlorodibenzo-p-dioxin alters the differentiation of alloreactive CD8+ T cells toward a regulatory T cell phenotype by a mechanism that is dependent on aryl hydrocarbon receptor in CD4+ T cells. J. Immunotoxicol. 5:81-91.

Garner MM, Ramsell K, Morera N, Juan-Sallés C, Jiménez J, Ardiaca M, Montesinos A, Teifke JP, Löhr CV, Evermann JF, Baszler TV, Nordhausen RW, Wise AG, Maes RK, Kiupel M (2008) Clinicopathologic features of a systemic coronavirus-associated disease resembling feline infectious peritonitis in the domestic ferret (Mustela putorius). Vet. Pathol. 45:236-46.

Ishmael JE, Löhr CV, Fischer K, Kioussi C (2008) Localization of myosin II regulatory light chain in the cerebral vasculature. Acta Histochem. 110:172-7.

Jin L, Löhr CV, Vanarsdall AL, Baker RJ, Moerdyk-Schauwecker M, Levine C, Gerlach RF, Cohen SA, Alvarado DE, Rohrmann GF (2008) Characterization of a novel alphaherpesvirus associated with fatal infections of domestic rabbits. Virology 378:13-20.

Jin L, Valentine BA, Baker RJ, Löhr CV, Gerlach RF, Bildfell RJ, Moerdyk-Schauwecker M (2008) An outbreak of fatal herpesvirus infection in domestic rabbits in Alaska. Vet. Pathol. 45:369-74.

Kioussi C and Gross MK (2008) How to Build Transcriptional Network Models of Mammalian Pattern Formation. PloS One 3:e2179.

Le UM, Yanasarn N, Löhr CV, Fischer KA, Cui Z (2008) Tumor chemo-immunotherapy using gemcitabine and a synthetic dsRNA. Cancer Biol. Ther. 7:440-7.

Marshall NB, Vorachek WR, Steppan LB, Mourich DV, Kerkvliet NI (2008) Functional characterization and gene expression analysis of CD4+ CD25+ regulatory T cells generated in mice treated with 2,3,7,8-tetrachlorodibenzo-p-dioxin. J. Immunol. 181:2382-91.

Robinson KM, Janes MS, and Beckman JS (2008) The selective detection of mitochondrial superoxide by live cell imaging. Nat Protoc. 3:941-7.

Siddens LK, Henderson MC, VanDyke JE, Williams DE and Krueger SK (2008) Characterization of mouse flavin-containing monooxygenase transcript levels in lung and liver, and activity of expressed isoforms. Biochem. Pharmacol. 755:570-9.

Sprague CR, Fraley TS, Jang HS, Lal S, and Greenwood JA (2008) Phosphoinositide binding to the substrate regulates susceptibility to proteolysis by calpain. J. Biol. Chem. 283, 9217-23.

Valentine BA and Löhr CV (2008) Myonecrosis in three horses with colic: evidence for endotoxic injury. Vet. Rec. 161:786-9.

Wang R, Dashwood WM, Löhr CV, Fischer KA, Nakagama H, Williams DE, and Dashwood RH (2008) beta-catenin is strongly elevated in rat colonic epithelium following short-term intermittent treatment with 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) and a high-fat diet. Cancer Sci. 99:1754-9.

Wang R, Dashwood WM, Löhr CV, Fischer KA, Pereira CB, Louderback M, Nakagama H, Bailey GS, Williams DE, and Dashwood RH (2008) Protective versus promotional effects of white tea and caffeine on PhIP-induced tumorigenesis and beta-catenin expression in the rat. Carcinogenesis 29:834-9.

2007

Andreasen EA, Mathew LK, Löhr C, Hasson R and Tanguay RL (2007) Aryl hydrocarbon receptor activation impairs extracellular matrix remodeling during zebrafish fin regeneration. Toxicol. Sci. 95:215-26.

Carter O, Dashwood RH, Wang R, Dashwood WM, Orner GA, Fischer KA, Löhr CV, Pereira CB, Bailey GS, and Williams DE (2007) Comparison of white tea, green tea, epigallocatechin-3-gallate, and caffeine as inhibitors of PhIP-induced colonic aberrant crypts. Nutr. Cancer 58:60-5.

Chung WG, Miranda CL and Maier CS (2007) Epigallocatechin gallate (EGCG) potentiates the cytotoxicity of rotenone in neuroblastoma SH-SY5Y cells. Brain Res. 1176:133-42.

Full SI, Deinzer MI, Ho PS, and Greenwood JA (2007) Phosphoinositide binding regulates alpha-actinin CH2 domain structure: analysis by hydrogen/deuterium exchange mass spectrometry. Protein Sci. 16:2597-604.

Indra AK, Castaneda E, Antal MC, Jiang M, Messaddeq N, Meng X, Loehr CV, Gariglio P, Kato S, Wahli W, Desvergne B, Metzger D and Chambon P (2007) Malignant transformation of DMBA/TPA-induced papillomas and nevi in the skin of mice selectively lacking RXRα in epidermal keratinocytes. J. Investigative Dermatol. 127:1250-60.

Kioussi C, Appu M, Löhr CV, Fischer KA, Bajaj G, Leid M and Ishmael JE (2007) Co-expression of myosin II regulatory light chain and the NMDAR1 subunit in neonatal and adult mouse brain. Brain Res. Bull. 74:439-51.

Mata JE, Dyal LA, Slausson M, Löhr CV, Summerton JE, Tyson AR and Gustafson SB (2007) Tumor imaging using technicium-99m bound to pH sensitive peptides. Nanomedicine 3:297-305.

Mathew LK, Sengupta S, Kawakami A, Andreasen EA, Löhr CV, Loynes, CA, Renshaw SA, Peterson RT and Tanguay RL (2007) Unraveling tissue regeneration pathways using chemical genetics. J. Biol. Chem. 282:35202-10.

Pehar M, Vargas MR, Robinson KM, Cassina P, Diaz-Amarilla PI, Hagen TM, Radi R, Barbeito L and Beckman JS (2007) Mitochondrial superoxide production and nuclear factor erythroid 2-related factor 2 activation in p75 neurotrophin receptor-induced motor neuron apoptosis. J. Neurosci. 27:7777-85.

Shaker DS, Sloat BR, Le UM, Löhr CV, Yanasarn N, Fischer KA and Cui Z (2007) Immunization by application of DNA vaccine onto a skin area wherein the hair follicles have been induced into anagen-onset stage. Mol. Ther. 15:2037-43.

Shih HP, Gross MK and Kioussi C (2007). Cranial muscle defects of Pitx2 mutants result from specification defects in the first branchial arch. Proc. Natl. Acad. Sci. 104:5907-12.

Shih HP, Gross MK and Koussi C (2007) Expression pattern of the homeodomain transcription factor Pitx2 during muscle development. Gene Expression Patterns 4:441-451.

2006

Cope RB, Loehr C, Dashwood R and Kerkvliet NI (2006) Ultraviolet radiation-induced non-melanoma skin cancer in the Crl:Skh:hr-1 hairless mouse: augmentation of tumor multiplicity by chlorophyllin and protection by indole-3-carbinol. Photochem. Photobio. Sci. 5:499-507.

Kioussi C, Shih HP, Loflin J and MK Gross (2006). Predictiing Active Nodes in the Transcriptional Network Governing Neural Tube Patterning. Proc. Natl. Acad. Sci. U S A 103:18621-26.

Pehar M, Vargas MR, Robinson KM, Cassina P, England P, Beckman JS, Alzari PM and Barbeito L (2006) Peroxynitrite transforms nerve growth factor into an apoptotic factor for motor neurons. Free Radic. Biol. Med. 41:1632-44.

Robinson KM, Janes MS, Pehar M, Monette JS, Ross MF, Hagen TM, Murphy MP and Beckman JS (2006) Selective fluorescent imaging of superoxide in vivo using ethidium-based probes. Proc. Natl. Acad. Sci. U S A 103:15038–43.

Smith-Roe SL, Löhr CV, Bildfell RJ, Fisher KA, Hegan DC, Glazer PM and Buermeyer AB (2006) Induction of aberrant crypt foci in DNA mismatch repair-deficient mice by the food-borne carcinogen 2-amino-1-methyl-6-phenylimidazo [4,5-b] pyridine (PhIP). Cancer Lett. 244:79-85.

Yu Z, Mahadevan B, Löhr CV, Fischer KA, Louderback MA, Krueger SK, Pereira CB, Albershardt DJ, Baird WM, Bailey GS and Williams DE (2006) Indole-3-Carbinol in the maternal diet provides chemoprotection for the fetus against transplacental carcinogenesis by the polycyclic aromatic hydrocarbon, dibenzo[a,l]pyrene. Carcinogenesis 27:2116-23.

Zhang Y, Vogel WK, McCullar JS, Greenwood JA and Filtz TM (2006) Phospholipase C-beta3 and -beta1 form homodimers, but not heterodimers, through catalytic and carboxyl-terminal domains. Mol Pharmacol. 70:860-8.